MFAP3L anticorps (AA 29-409)

N° du produit ABIN7601132

Cet anticorps anti-MFAP3L est un anticorps Lapin Polyclonal détectant MFAP3L dans ELISA, WB, IF et ICC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour MFAP3L anticorps (AA 29-409) (ABIN7601132)

Antigène: MFAP3L (Microfibrillar-Associated Protein 3-Like (MFAP3L))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MFAP3L est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-MFAP3L anticorps

Épitope: AA 29-409

Fonction: Anti-MFAP3L Antibody Picoband®

Attributs du produit: Anti-MFAP3L Antibody Picoband® (ABIN7601132). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MFAP3L recombinant protein (Position: K29-V409). Human MFAP3L shares 90.6% amino acid (aa) sequence identity with both mouse and rat MFAP3L.

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998. 2. Lou, X., Kang, B., Zhang, J., Hao, C., Tian, X., Li, W., Xu, N., Lu, Y., Liu, S. MFAP3L activation promotes colorectal cancer cell invasion and metastasis. Biochim. Biophys. Acta 1842: 1423-1432, 2014.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur MFAP3L

Antigène: MFAP3L (Microfibrillar-Associated Protein 3-Like (MFAP3L))

Autre désignation: MFAP3L

Sujet: Microbibrillar-Associated Protein 3-Like (MFAP3L), also known as NYD-sp9, is part of the microfibrillar-associated protein family (MFAPs). MFAPs are non-fibrillin, extracellular matrix glycoproteins that interact with fibrillin and were originally characterized in microfibrillar assembly. In humans, there several subfamily members with varying amino acid (aa) sequence homology and functions . Among the family, MFAP2 and MFAP5 are more closely related and while MFAP1, 3 and 4 share no structural or sequence homology with MFAP2, MFAP5 or with each other. Human MFAP3L shows 71 % amino acid (aa) sequence homology to MFAP3, but not other MFAPs. Mature, human MFAP3L consists of an extracellular domain (ECD) containing N-linked glycosylation sites, a transmembrane domain, and a cytoplasmic domain with a conserved SH2 motif. The ECD of human MFAP3L shares 89 % and 90 % aa sequence identity with mouse and rat MFAP3L, respectively. MFAPs have the unique ability to interact with TGF-beta family growth factors, Notch and Notch ligands and multiple elastic fiber proteins, in addition to interacting with fibrillin. MFAPs are expressed in a wide variety of tissues and, along with microfibril assembly, they play roles in the regulation of tissue homeostasis, cell survival, and tumor progression. MFAP3L is often located within colorectal cancer (CRC) cells, which metastasize by activation of the nuclear ERK pathway via MFAP3L phosphorylation. Regulation of this MFAP3L activity could have pharmaceutical effects on CRC tumor progression.

Poids moléculaire: 45 kDa

ID gène: 9848

UniProt: O75121